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发布于:2018-11-3 22:38:24  访问:49 次 回复: 篇
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Average mean, median and mode values for the sizing of 767 diploid
Size mutant failures: Statistical analysis for cell size of your 14 mutants which failed to repeat the size phenotypes. Repeatability: Comparative cell size analysis from the overlapping 144 newly constructed deletion strains using the preceding screens. Epistasis size values: Logarithmic phase mean cell size readings for the epistatic combinations. More file two: Figure S1. Cell size analyses of yeast deletion strains. (A) Total of 767 deletion strains (homozygous+heterozygous) were sized within the logarithmic phase. Each and every data point represents the amount of strains whose size falls in a five fL bin. The two curves represent the geometric imply (EED226 Epigenetics diamond) and median (square) in the cell sizes. Upper and decrease size limits are indicated by dashed lines and 95 of strains had mean cell sizes inside the selection of 82.7fL and 118fL (?SD from the typical mean cell size). WT imply cell size (104.1fL) is depicted by the arrow. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25112874 (B) Total of 772 deletion strains (homozygous+heterozygous) had been sized within the saturation phase. Each and every information point represents the number of strains whose size falls inside a 5 fL bin. The two curves represent the geometric imply (diamond) and median (square) of the cell sizes. Upper and decrease size limits are indicated by dashed lines and 95 had mean cell sizes inside the range of 38.8fL and 62.8fL (?SD on the typical imply cell size). WT imply cell size (48.8fL) is depicted by the arrow. Extra file 3: Figure S2. Cell size distribution curves in saturation phase. Geometric mean distribution curves are represented for the strainsDungrawala et al. Cell Division 2012, 7:24 http://www.celldiv.com/content/7/1/Page 12 of17. Dolznig H, Grebien F, Sauer T, Beug H, Mullner EW: Evidence to get a sizesensing mechanism in animal cells. Nat Cell Biol 2004, six:899?05. 18. Grebien F, Dolznig H, Beug H, Mullner EW: Cell size control: new evidence for any general mechanism. Cell Cycle 2005, four:418?21. 19. Cooper S: Control and maintenance of mammalian cell size. BMC Cell Biol 2004, 5:35. 20. Conlon I, Raff M: Differences inside the way a mammalian cell and yeast cells coordinate cell growth and cell-cycle progression. J Biol 2003, two:7. 21. Nurse P, Thuriaux P, Nasmyth K: Genetic manage from the cell division cycle in the fission yeast Schizosaccharomyces pombe. Molecular basic genetics: MGG 1976, 146:167?78. 22. Nash R, Tokiwa G, Anand S, Erickson K, Echinocystic acid Futcher AB: The WHI1+ gene of Saccharomyces cerevisiae tethers cell division to cell size and is really a cyclin homolog. EMBO J 1988, 7:4335?346. 23. Cross FR: DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae. Mol Cell Biol 1988, eight:4675?684. 24. Nurse P: Genetic manage of cell size at cell division in yeast. Nature 1975, 256:547?51. 25. Tyers M, Tokiwa G, Nash R, Futcher B: The Cln3-Cdc28 kinase complex of S. cerevisiae is regulated by proteolysis and phosphorylation. EMBO J 1992, 11:1773?784. 26.Typical imply, median and mode values for the sizing of 767 diploid strains in PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/25112874 the logarithmic phase (299 homozygous diploids + 468 heterozygous diploids).
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